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KMID : 1140120120170040315
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Cancer Prevention Research
2012 Volume.17 No. 4 p.315 ~ p.323
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Anti-metastatic Effects of Genistein in HCT116 Colon Cancer Cellsvia Inhibition of COX-2
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Bae Woo-Ri
Park Song-Yi
Lee Sol-Hwa
Kim In-Seop
Park Ock-Jin
Kim Young-Min
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Abstract
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COX-2 is known to play an important role in cell metastasis and angiogenesis, and COX-2 is also over-expressed in colon cancer cells. In this study, we explored the anti-metastatic effects of genistein in HCT116 colon cancer cells, mediated effects on COX-2. Genistein treatments at different concentrations and for different durations inhibited proliferation and migration of HCT116 colon cancer cells. Genistein treatments decreased COX-2, MMP-9, Ang-1, VASP and VEGF. Treatment with celecoxib (an COX-2 inhibitor) decreased migration of HCT116 cells, while a combined treatment with celecoxib and genistein resulted in even futher decrease in cell migration. Inhibition of COX-2 by celecoxib treatment abolished these inhibitory effects on cell metastasis. COX-2 inhibition decreaesed MMP-9 expression in HCT116 cells, while a combined treatement with celecoxib and genistein resulted in even further decresed expression of MMP-9 in HCT116 cells. These results suggest that genistein induced both anti-proliferative and anti-metastatic effects by inhibiting COX-2 and MMP-9 activities. Genistein treatment also appeared to induce synergistic anti-metastatic effects by inhibition of COX-2 in HCT116 colon cancer cells.
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KEYWORD
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Genistein, Anti-metastatic effect, HCT116 colon cancer cells, COX-2, Celecoxib
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